6-Benzylaminopurine 98% TC Usage Guide | 6-BA Cytokinin
Introduction： What is 6-Benzylaminopurine (6-BA)？
6-Benzylaminopurine（6-BA） is a first-generation synthetic cytokinin plant growth regulator widely used in modern agriculture to enhance plant growth, development and yield. Structurally related to adenine，6-BA mimics the function of natural cytokinins to stimulate cell division，differentiation and other key physiological processes in plants.
As a broad-spectrum plant growth regulator，6-BA can be applied throughout the entire crop growth cycle，from seed germination to post-harvest preservation. It is valued for its high efficiency, low cost and excellent safety profile.
Mode of Action： How 6-BA Works on Plants
6-BA functions by promoting cell division in plant meristems（root tips，shoot tips，cambium），stimulating the synthesis of proteins，nucleic acids and chlorophyll. Key physiological effects include：
-
Promoting cell division and differentiation：6-BA accelerates cell division in shoot and root meristems，leading to vigorous plant growth.
-
Delaying senescence：By inhibiting the decomposition of chlorophyll，proteins and nucleic acids，6-BA keeps leaves green and photosynthetically active longer.
-
Breaking apical dominance：6-BA stimulates lateral bud growth，resulting in bushier plants with more branches and flowers.
-
Enhancing nutrient transport：It facilitates the movement of amino acids，auxins and minerals to treated sites，improving fruit set and quality.
Key Benefits of 6-Benzylaminopurine 98% TC
| Benefit | Description |
|---|---|
| Stimulates cell division | Promotes vigorous shoot and root growth，enhancing overall plant vigor. |
| Breaks bud dormancy | Effectively breaks dormancy in seeds and buds，accelerating uniform germination and emergence. |
| Promotes lateral branching | Suppresses apical dominance to encourage lateral bud break，resulting in fuller, bushier plants with improved canopy coverage. |
| Delays leaf senescence | Keeps foliage green and functional longer，improving photosynthetic efficiency and delaying maturity where desired. |
| Improves fruit set and quality | When applied at flowering，6-BA reduces fruit drop and increases fruit size，firmness and soluble solids content. |
| Enhances post-harvest shelf life | Extends the green life of harvested vegetables and cut flowers. |
| Residue-safe | Maximum usage of 0.01 g/kg ensures residue levels below 0.2 mg/kg，making it suitable for export-oriented produce. |
Application Methods for 6-BA Cytokinin
The following application methods are recommended for 6-Benzylaminopurine 98% TC：
Foliar Spray（Most Common）
-
Best for：General growth promotion，branching，fruit set
-
Advantages：Rapid absorption through leaves，quick response
-
Timing：Apply during active growth periods，targeting young leaves and shoots
Seed Treatment（Soaking）
-
Best for：Improving germination rates and early vigor
-
Typical rate：20 mg/L for 6 hours to enhance germination
-
Ensure uniform coverage of seeds
Bud/Local Application
-
Best for：Stimulating lateral buds on woody plants（apple trees，roses）
-
Make small incisions 0.5 cm above and below the axillary bud site and apply the solution directly with a brush
Soil Drench
-
Suitable for certain crops as an alternative to foliar spray
Dilution and Usage Guidelines （By Crop Type）
The following recommended concentrations are based on comprehensive research and agricultural field trials：
5.1 Fruit Trees and Orchards
| Crop | Purpose | Concentration | Method | Notes |
|---|---|---|---|---|
| Apple | Stimulate lateral branching | 100–500 ppm（mg/L） | Foliar spray to actively growing shoots | Apply during vigorous growth for branching |
| Apple | Fruit thinning | 30–75 g/acre（about 33–83 ppm） | Foliar spray after bloom | Improves fruit size and firmness |
| Citrus | Flower bud differentiation and fruit set | 100–133 ppm（7,500–10,000× dilution） | Foliar spray | Enhances fruit set and reduces drop |
| Jujube | Fruit set | 100–200 ppm（5,000–10,000× dilution） | Foliar spray at flowering | |
| Young apple trees （shaping） | Lateral bud sprouting | 300–400 ppm（3% solution diluted 75–100×） | Spray buds during vigorous growth | For branch formation |
5.2 Grapes
| Purpose | Concentration | Method |
|---|---|---|
| Break bud dormancy | 33.3–50 mg/L（33–50 ppm） | Soak dormant buds or shoots before planting |
| Seedless fruit development（with GA3） | 33.3–50 mg/L 6-BA ＋ 100–200 mg/L GA3 | Dip inflorescences when 95% of flowers are open |
| Prevent flower/fruit drop | 100 mg/L | Apply to grape inflorescences 2 weeks before flowering |
💡Note：6-BA increases cell number in grape berries，resulting in larger fruit independent of thinning effects.
5.3 Field Crops： Rice, Wheat, Barley
| Crop | Purpose | Concentration | Method |
|---|---|---|---|
| Rice, wheat, barley | Increase weight and yield | 10–20 ppm | Foliar spray |
| Rice | Weed control in pre-emergence | 0.05–1 mg/L | Seed soaking, pre-emergence |
| Corn | Increase leaf thickness, improve photosynthesis | Mixed with ethephon | Foliar application during vegetative stage |
5.4 Vegetables and Cash Crops
| Crop | Purpose | Concentration | Method |
|---|---|---|---|
| Cucumber | Fruit enlargement | 50–100 ppm | Dip fruit 2–3 days after flowering |
| Lettuce, cabbage, cauliflower | Post-harvest preservation | 100–500 mg/L | Spray or soak before/after harvest |
| Potato | Minituber formation | N/A | Pre-planting treatment to enhance shoot，stolon and root development |
| Groundnut（peanut） | Increase branching and pod yield | N/A | Seed pre-treatment with BAP；also prepones flowering |
5.5 Flowers and Ornamentals
| Plant | Purpose | Concentration | Method |
|---|---|---|---|
| Roses | Basal shoot formation | 0.5% ointment or solution | Apply to incisions 0.5 cm above/below buds |
| Cyclamen，cacti | Promote flowering | N/A | Apply during bud formation stage |
| Spinach | Uniform bolting and seed production | N/A | Timing－dependent application |
5.6 Tissue Culture Applications
For in vitro plant propagation，6-BA（often referred to as 6-BAP in lab settings） is a “laboratory favorite” cytokinin. Use 0.1–10 mg/L in culture media depending on species and desired shoot multiplication response.
💡Pro Tip：For tissue culture，prepare a 1 mg/mL stock solution by dissolving 100 mg 6-BA in a minimal volume of 0.1M NaOH or HCl，then bring to 100 mL with distilled water. Sterilize by filtration（0.22 µm） before adding to autoclaved media.
How to Dilute 6-BA 98% TC Powder Correctly
Because 6-BA has low water solubility（approx. 0.1 g/L at 20°C），special pre-dissolution steps are required.
✅Step-by-Step Dilution Method：
-
Weigh the desired amount of 6-BA 98% TC powder.
-
Pre-dissolve in a small amount of 0.1 mol/L hydrochloric acid（HCl） OR dilute acetic acid OR 70% ethanol.
-
Once fully dissolved，gradually add water to the final volume while stirring continuously（warm water at 40–50°C can aid dissolution）.
-
Adjust pH to 6.0–7.0 if necessary，as 6-BA remains stable across a broad pH range but is most effective when applied at near-neutral pH.
🌾Example calculation：To prepare 20 L of 200 ppm（mg/L） 6-BA solution：
200 mg/L × 20 L = 4,000 mg = 4 g of 6-BA 98% TC powder
Dissolve 4 g of powder in 50–100 mL 0.1M HCl，then dilute with water to 20 L.
Best Practices for Foliar Spray Application
For optimal efficacy when using 6-Benzylaminopurine as a foliar spray：
| Factor | Recommendation |
|---|---|
| Application timing | Early morning or late afternoon（avoid midday heat） |
| Water quality | Use clean water with pH 5.0–7.0；performance decreases above pH 8.5 |
| Spray coverage | Apply as a uniform fine mist；direct 85% of spray volume to upper two-thirds of canopy（for trees） |
| Adjuvants | Non-ionic surfactants may improve leaf wetting and penetration |
| Do NOT apply to | Plants under stress（drought，disease，winter injury） or low-vigor trees |
| Reapplication interval | 10–14 days as needed during active growth stages |
Combination with Other Growth Regulators
6-BA shows synergistic effects when combined with other plant growth regulators：
6‑BA ＋ Gibberellic Acid（GA3）
-
Fruit enlargement：6-BA combined with GA3 can increase yield by more than 30%，and in some crops up to 60%.
-
Seedless fruit production（grapes）：33.3–50 mg/L 6-BA ＋ 100–200 mg/L GA3.
-
Apple fruit thinning & sizing：6-BA increases cell number in fruit independent of thinning，resulting in larger size and improved firmness and soluble solids.
6‑BA ＋ Ethephon
-
Corn（maize）：Increases leaf thickness，makes plants more compact，improves photosynthesis efficiency，enhances lodging resistance and prevents premature aging，ultimately increasing corn yield.
6‑BA ＋ Auxins（NAA/IBA）
-
Tissue culture：Auxin∶cytokinin ratios control root vs shoot differentiation；with 6‑BA promoting shoots and auxins promoting roots.
Safety and Handling Guidelines
6-Benzylaminopurine has an excellent safety profile for agricultural use：
| Parameter | Value |
|---|---|
| Acute oral LD50（rat, male） | 2,125 mg/kg |
| Acute oral LD50（rat, female） | 2,130 mg/kg |
| Acute oral LD50（mouse） | 1,300 mg/kg |
| Toxicity classification | Low toxicity |
| Skin/Eye irritation | Moderate eye irritant |
🛡️Safety precautions when handling 6-BA 98% TC：
-
Wear protective gloves，goggles and dust mask when handling the powder.
-
Avoid inhalation of dust and direct contact with eyes or skin.
-
Wash thoroughly after handling.
-
Store in a cool，dry place away from direct sunlight，food and feed.
-
Keep out of reach of children.
-
Dispose of empty containers according to local regulations.
🌱Residue profile：Following recommended application rates（e.g. maximum usage 0.01 g/kg），residue levels remain below 0.2 mg/kg，well within global maximum residue limits（MRLs）. This makes 6-BA suitable for use on export-oriented crops.
Conclusion
6-Benzylaminopurine 98% TC（6-BA） is a highly effective，cost‑efficient cytokinin plant growth regulator suitable for fruit trees（apple，citrus，jujube），grapes，field crops（rice，wheat，barley，corn），vegetables（cucumber，lettuce，potato），flowers and ornamentals，as well as tissue culture applications.
✅Key takeaways：
-
Promotes cell division，lateral branching and uniform flowering/fruiting
-
Enhances fruit quality（size，firmness，soluble solids）
-
Delays senescence and extends post-harvest shelf life
-
Excellent safety profile（low mammalian toxicity） and favorable residue levels
For optimal results：
-
Pre‑dissolve 6-BA powder in 0.1M HCl or ethanol before dilution.
-
Apply at recommended concentrations（10–500 ppm depending on crop and purpose）.
-
Time applications according to crop phenology（e.g.，post‑bloom for fruit trees，2 weeks pre‑flowering for grapes）.
-
Use clean water with pH 5.0–7.0；avoid spraying plants under stress.
-
Consider synergistic combinations with GA3 or ethephon for enhanced effects.
📧Contact our sales team for a quote：
👉 Click here to send an inquiry
🏭Factory direct supply｜High purity 98% TC｜Stable quality｜Low factory cost｜Fast delivery worldwide